Column chromatography
Chromatography is a separation technique that separates the ink from the dyes.
Column chromatography is used to extract a single chemical compound from a mixture. It consists of a stationary phase (solute which is silica or alumina gel) and a mobile phase (a liquid solvent), eluent (added at the top of the mixture), column (glass tube that contains the stationary phase) and fractions (the samples of isolated compounds that are gathered at the bottom of the column using test tubes).
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The reaction works based on how the stationary phase interacts with the components that either binds weakly to the column moving quickly through the column to the bottom of it or binds strongly to the column moving slowly through the column.
Column chromatography is useful in pharmaceutical science such as drug discovery and purifying natural products and microorganisms, forensics such as separating chemical compounds from crime investigations and in biotechnology such as purifying proteins and macromolecules.
Fig 1; the image showing the representation of the parts column chromatography
