GetX

We developed GetX, a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. GetX allows the user to design synthetic DNA fragments required to perform bacterial genome editing with simple inputs of 1) genome information from the target strain and 2) information for the desired mutation. GetX designs DNA fragments for introduction of sequences for protein tagging, deletions, insertions, and point mutations. It also designs synonymous codon fragments required to manipulate essential target genes to enable survival of the strain during the genome editing procedure. GetX provides genbank and fasta format files for a mutation template plasmid, a mutation cassette, and a sequence around the mutation site to guide the user through the design of the materials required for the scarless bacterial genome editing method.